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KMID : 0545120000100010115
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.115 ~ p.118
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Expression of Enhanced Green Fluorescent Protein from Stably Transformed Drosophila melanogaster S2 Cells
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LEE, JONG MIN
PARK, JONG HWA/CHUNG, IN SIK
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Abstract
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Recombinant plasmids harboring a heterologous gene coding for the enhanced green fluorescent protein (EGFP) were transfected and expressed in Drosophila melanogaster S2 cells. A stable transformation of polyclonal cell populations expressing EGFP were isolated after 4 weeks of selection with hygromycin B. The recombinant EGFP expressed in transformed S2 cells consisted of a molecular weight of 27kDa. EGFP expression was also confirmed by fluorometric measurement. The maximum EGFP concentration was about 9.3㎎/l. The present findings demonstrate not only the successful stable expression of EGFP in Drosophila S2 cells, but also the use of EGFP as a reporter to analyze gene expression, with its potential of a Drosophila cellexpression system for recombinant protein production being an alternative to a baculovirus-insect cell expression system.
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